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Appendix I.
Extermination of Plague Bacilli in Vermin.
Honourable COLONIAL SECRETARY,
I should like to know how long bugs, fleas, spiders, &c., will live in a 5 per cent. solution of Jeyes' fluid. This affects the question of disinfection, for as the plague bacillus is found in these vermin it is evident that no disinfection that does not kill the vermin will reach the bacillus.
H. A. B.
14-7-03.
GOVERNMENT PUBLIC MORTUARY, 11th August, 1903,
SIR,- have the honour to submit the following Report on the question as to how long bugs survive in a solution of Jeyes' Fluid, for the information of His Excellency the Governor, Attached is a detailed account of the experiments per-
formed.
From these experiments I venture to draw the following conclusions :-
1. It is obvious that Jeyes Fluid of such a concentration as used in Series
A and B is not of auch practical value.
2. The killing power of Jeyes'/Fluid as employed in Series C and D) is
uncertain, even after 15 minutes.
3. If bugs are to be exterminated with certainty, it is apparent that the concentration of Jeyes Fluid as employed in Series L'and I'must
The Honourable
be used.
J. M. ATKINSON, M.B.,
A. SERIES.
I have the honour to be
Principal Civil Medical Officer,
ye.
ge.,
Sir,
Your obedient Servant,
WILLIAM HUNTER.
After removal from the solution, the animals appeared to be dead.
After
THE ACTION OF JEYES' FLUID ON Buns.
1 Part or 1 c.c. of Jeyes' Fluid in 100 c.c. of water.
Experiment No. 1.--2 bugs completely immersed for 1 minute.
5 minutes, however, movements of the legs began which were followed 10 minutes later by complete recovery of both aunals.
Experiment No. 2.-2 bugs completely immersed for 2 minutes.
The same phenomena were observed as in Experiment No. 1. Complete
recovery of both animals followed in 15 minutes.
Experiment No. 3.—2 bugs completely immersed for 5 minutes.
The same phenomena were observed as before.
lively in about 15 minutes.
Both animals were quite
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Experiment No. 4.—2 bugs completely immersed for 15 minutes.
After removal from the solution, the animals appeared to be dead and
remained in that condition for about 20 minutes.
Complete recovery of both animals took place in about 45 minutes after
removal from the solution.
In connection with this (4. Series of Experiments) a few interesting phenomena were noted.. Immediately on immersion. the animals shewed vigorous movements of the legs and moved about actively in the fluid. This, in a few seconds, is succeeded by a few convulsive efforts in all the extremities contiming from 1 to 2 seconds, Subsequently all movements ceased.
Ou expiration of the time limit of the particular experiment, the animals were transferred to a clean, dry glass capsule and frequently examined by a low power miscroscopic objective. For some considerable time no movements on the part of the animal were detected, eg, Haperi- ment No. L-For 5 minutes. At the expiration of this time slight convulsive seizures in the body and extremities commenced; these became gradually more pronounced and eventually more purposive until the animals completely recovered.
Washing the animals in water after immersion in Jeyes' Fluid solution or
exposure to the sun accelerates their recovery.
B.--SERIES.
2 Parts or 2 c.c. of Jeyes' Fluid in 100 e.c. of water.
Experiment No. 1-2 bugs completely immersed for 1 minute.
After removal, the animals appeared to be dead. Movements of the ex-
tremities commenced in about 10 minutes.
Both animals completely recovered in 20 minutes.
Experiment No. 2.-2 bugs completely immersed for 5 minutes.
After removal, animals remained motionless.
Muscular movements commenced in 15 minutes.
Both animals recovered in 30 mimates,
Experiment No. 3.--2 bugs completely immersed for 15 minutes.
After removal of the animals, the same phenomena were observed. Muscular movements commenced in 15 minutes. Both animals completely recovered in 35 minutes.
C.-SERIES.
3 Parts or 3 c.c. Jeges' Fluid in 100 c.c. of water.
Experiment No. 1.--2 bugs completely immersed for 1 miunte,
On removal, same phenomena as before.
Muscular movements began in 15 minutes.
Complete recovery of both animals in 45 minutes.
Experiment No. 2.--2 bugs completely immersed for 5 minutes.
Ön removal, same appearances met with as in previous experiments.
Muscular movements began in 30 minutes, in one animal. This bug
was quite lively after 1 hour.
The other animal was dead.
Experiment No. 3.--2 bugs completely immersed for 5 minutes.
Both animals were dead on removal from the solution.
The observation extended over 4 hours.
This series of experiments was repented with almost similar results.
.-SERIES.
D.-
4 Parts or 4 cc. of Jeyes' Fluid in 100 c.c. of water.
Experiment No. 1.-2 bugs completly immersed for 1 minute.
After removal, the same phenomena were observed as before. Both animals were quite lively in 10 minutes.
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